2X6G
X-ray Structure of Macrophage Inflammatory Protein-1 alpha (D27A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2001-11-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.211, 113.527, 173.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.012 - 2.180 |
| R-factor | 0.2143 |
| Rwork | 0.210 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 61457 | |
| <I/σ(I)> | 31.6 | 4.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NH4AC, 0.1M HEPES (PH7.8), 26% PEG3350 |
