2X65
Crystal structure of T. maritima GDP-mannose pyrophosphorylase in complex with mannose-1-phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.933, 91.738, 69.730 |
| Unit cell angles | 90.00, 110.75, 90.00 |
Refinement procedure
| Resolution | 65.210 - 2.100 |
| R-factor | 0.18837 |
| Rwork | 0.187 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x5s |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.200 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 43388 | |
| <I/σ(I)> | 17.3 | 2.5 |
| Completeness [%] | 98.7 | 98.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 35% (V/V) MPD |
