2X60
Crystal structure of T. maritima GDP-mannose pyrophosphorylase in complex with GTP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.926, 79.567, 70.952 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 67.570 - 2.800 |
| R-factor | 0.21104 |
| Rwork | 0.207 |
| R-free | 0.26697 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x5s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.600 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 17147 | |
| <I/σ(I)> | 16 | 2.6 |
| Completeness [%] | 98.9 | 99.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 100 MM SODIUM ACETATE PH 5.0, 30% (V/V) MPD, 200 MM CALCIUM CHLORIDE |
