2X5S
Crystal structure of T. maritima GDP-mannose pyrophosphorylase in apo state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.011, 92.999, 69.687 |
| Unit cell angles | 90.00, 110.25, 90.00 |
Refinement procedure
| Resolution | 65.370 - 2.350 |
| R-factor | 0.22648 |
| Rwork | 0.224 |
| R-free | 0.27315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cu2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.154 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.370 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.055 | 0.470 |
| Number of reflections | 31744 | |
| <I/σ(I)> | 23.5 | 2.5 |
| Completeness [%] | 99.2 | 96.9 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 100 MM PCB PH 7.5, 35% (V/V) MPD |
