2X5I
Crystal structure echovirus 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 297.100, 297.700, 300.600 |
| Unit cell angles | 119.00, 100.10, 108.40 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| R-factor | 0.2882 |
| Rwork | 0.288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cov |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | GLRF |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.240 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.090 | 0.280 |
| Number of reflections | 655131 | |
| <I/σ(I)> | 5.95 | 1.98 |
| Completeness [%] | 45.9 | 32.3 |
| Redundancy | 1.2 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP, BOTTOM SOLUTION CONTAINED 0.25% PEG 8000, 0.25% GLYCEROL, 400 MM NACL, 150 MM CACL, 100 MM TRIS PH 8.0 |
