2X5G
Crystal structure of the ORF131L51M mutant from Sulfolobus islandicus rudivirus 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.453, 58.453, 68.113 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.311 - 2.000 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.24890 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.310 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 9346 | |
| <I/σ(I)> | 20.9 | 1.7 |
| Completeness [%] | 99.0 | 92 |
| Redundancy | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M SODIUM MALONATE AND CRYOPROTECTED WITH 2.4 M MALONATE |
