2X58
The crystal structure of MFE1 liganded with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.700, 126.500, 227.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 2.800 |
| R-factor | 0.22679 |
| Rwork | 0.224 |
| R-free | 0.28196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ZCJ 1wdk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.581 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.660 |
| Number of reflections | 46656 | |
| <I/σ(I)> | 11.5 | 2.6 |
| Completeness [%] | 98.1 | 98 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100MM MES PH 6.0, 150MM AMMONIUM SULPHATE, 15% W/V PEG4000 |
