2X57
Crystal structure of the extracellular domain of human Vasoactive intestinal polypeptide receptor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9809, 0.9799 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.700, 99.700, 104.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.20844 |
| Rwork | 0.207 |
| R-free | 0.23477 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.410 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.870 |
| Number of reflections | 31377 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 40% PEG300 0.1M CITRATE PHOSPHATE PH4.2 |
