2X56

Yersinia Pestis Plasminogen Activator Pla (Native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	140.241, 140.241, 130.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.300
R-factor	0.201
Rwork	0.199
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PLA NATIVE OSMIUM DERIVATIVE
RMSD bond length	0.007
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.100
Number of reflections	31644
<I/σ(I)>	31
Completeness [%]	99.4
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.8		27% PEG 400, 0.1 M LISO4, 0.1 M LI CITRATE, PH 4.8