2X53
Structure of the phage p2 baseplate in its activated conformation with Sr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 300.278, 239.511, 274.782 |
| Unit cell angles | 90.00, 124.36, 90.00 |
Refinement procedure
| Resolution | 39.310 - 3.900 |
| R-factor | 0.2296 |
| Rwork | 0.229 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 4.100 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.160 | 0.480 |
| Number of reflections | 141311 | |
| <I/σ(I)> | 5.4 | 2.3 |
| Completeness [%] | 97.0 | 97.5 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20C, PROTEIN 4 MG/ML. 3.3% PEG 8000 IN 33 MM TRIS PH 8.5, 130 MM SR ACETATE, 66 MM LI SULFATE, AND 66 MM IMIDAZOLE. |
