2X51

M6 delta Insert1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-11
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	163.888, 61.017, 133.274
Unit cell angles	90.00, 116.19, 90.00

Refinement procedure

Resolution	26.818 - 2.200
R-factor	0.2186
Rwork	0.216
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bkh
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.070
Number of reflections	60348
<I/σ(I)>	14.44	3.92
Completeness [%]	99.7	99.9
Redundancy	4.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.75		10.8% PEG 8000, 50 MM MES PH 6.75, 175 MM AMMONIUM SULFATE, 3% ISO-PROPANOL, 3% TERT-BUTANOL, 4 MM EGTA. SOLUTION OF PROTEIN AT 18 MG/ML.