2X4Q
Crystal structure of MHC CLass I HLA-A2.1 bound to a photocleavable peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.030, 86.486, 80.466 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 32.434 - 1.900 |
| R-factor | 0.1693 |
| Rwork | 0.167 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 64908 | |
| <I/σ(I)> | 7.9 | 1.1 |
| Completeness [%] | 97.2 | 95.2 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH 6.5, 20% PEG1500 |
