2X4P
Crystal structure of MHC CLass I HLA-A2.1 bound to a photocleavable peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.201, 86.496, 80.783 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 19.822 - 2.300 |
| R-factor | 0.1614 |
| Rwork | 0.158 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 37987 | |
| <I/σ(I)> | 4.7 | 1.7 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 20% PEG1500 |
