2X4H
Crystal Structure of the hypothetical protein SSo2273 from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-27 |
| Detector | FUJIFILM |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.100, 72.100, 260.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 2.300 |
| R-factor | 0.20438 |
| Rwork | 0.202 |
| R-free | 0.24625 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCDE (SOLVE RESOLVE) |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.890 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 30005 | |
| <I/σ(I)> | 23.8 | 4.8 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5.6%-6.8% PEG 1000, 80MM ZN ACETATE AND 0.1M MES PH7. THE CRYSTALS WERE CRYOPROTECTED BY ADDITION OF 25% PEG 400. |
