2X4A
Crystal structure of the C-terminal domain of InvA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-26 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.170, 79.170, 127.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.730 - 2.540 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x49 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.812 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 15561 | |
| <I/σ(I)> | 12.6 | 4 |
| Completeness [%] | 96.5 | 76.2 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 0.2 M LISO4, 0.1 M CAPS PH 10.5, 1.2 M NAH2PO4/0.8M K2HPO4 |
