2X3X
structure of mouse syndapin I (crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.077, 154.545, 255.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.130 - 3.350 |
| R-factor | 0.2234 |
| Rwork | 0.221 |
| R-free | 0.27701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.170 | 3.450 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.120 | 0.860 |
| Number of reflections | 23772 | |
| <I/σ(I)> | 14.97 | 2.62 |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 6.11 | 6.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 2.5UL SYNDAPIN (5MG/ML IN 50MM HEPES, 50MM NACL) MIXED WITH 0.5UL 0.3M GLYCYL-GLYCYL-GLYCINE AND 2UL WELL SOLUTION 0.1M NAAC/HAC PH5.3, 3%(W/V) PEG4000. |
