2X3U
Ferredoxin-NADP reductase mutant with Tyr 303 replaced by Phe (Y303F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Collection date | 2008-06-29 |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.245, 86.245, 96.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.350 - 1.930 |
| R-factor | 0.17544 |
| Rwork | 0.173 |
| R-free | 0.20526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.440 | 2.010 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 28671 | |
| Completeness [%] | 93.9 | 88.2 |
| Redundancy | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % PEG 6000, 0.1 M NAAC PH 5.5, 20 MM (NH4)2SO4 |
