2X3O
Crystal Structure of the Hypothetical Protein PA0856 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.440, 51.740, 68.800 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 29.140 - 2.900 |
| R-factor | 0.26472 |
| Rwork | 0.262 |
| R-free | 0.31584 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.140 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.860 |
| Number of reflections | 10798 | |
| <I/σ(I)> | 14.97 | 2.22 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 36.3% PEGMME 550, 0.1M SODIUM CACODYLATE PH 6.5, 0.16M DI-AMMONIUM PHOSPHATE. CRYSTALS WERE CRYOPROTECTED WITH 3.67% HEXANEDIOL IN THE ABOVE SOLUTION |
