2X3M
Crystal Structure of Hypothetical Protein ORF239 from Pyrobaculum Spherical Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.898, 53.715, 95.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.560 - 1.450 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.916 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0079) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.500 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.460 |
| Number of reflections | 39560 | |
| <I/σ(I)> | 28.3 | 1.8 |
| Completeness [%] | 97.0 | 73 |
| Redundancy | 4.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.2M POTASSIUM ACETATE CRYOPROTECTION WITH 20% PEG 400 |
