2X3E
Crystal structure of 3-oxoacyl-(acyl carrier protein) synthase III, FabH from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 69.240, 69.240, 212.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 213.200 - 1.810 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.20377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.078 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0090) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 49430 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 99.0 | 90 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 2.8M DI-AMMONIUM-PHOSPHATE, 0.1M SODIUM CITRATE, PH4.5, 120MM AMMONIUM ACETATE.CRYOPROTECTED WITH 3.6M DI-AMMONIUM-PHOSPHATE, 50MM NACITRATE, PH4.5, 100MM AMMONIUM ACETATE |
