2X3B
AsaP1 inactive mutant E294A, an extracellular toxic zinc metalloendopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.855, 60.196, 183.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.810 - 2.280 |
| R-factor | 0.24673 |
| Rwork | 0.244 |
| R-free | 0.29332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x3c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.600 |
| Number of reflections | 33551 | |
| <I/σ(I)> | 19.3 | 2.69 |
| Completeness [%] | 99.4 | 93.1 |
| Redundancy | 6.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% PEG 4000, 0.2 M (NH2)4SO4, 0.1 M HEPES PH 7.5, 10% ISO-PROPANOL |
