2X2I
Crystal structure of the Gracilariopsis lemaneiformis alpha-1,4- glucan lyase with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 112 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-12 |
| Detector | MACSCIENCE DIP2030H |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.861, 91.804, 193.349 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 41.850 - 2.600 |
| R-factor | 0.23385 |
| Rwork | 0.229 |
| R-free | 0.32040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.275 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.230 | 0.640 |
| Number of reflections | 118187 | |
| <I/σ(I)> | 4.6 | 1 |
| Completeness [%] | 82.8 | 79.3 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18-21% PEG 8000, 0.1M SODIUM ACETATE, PH 5.0-5.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
