2X1Z
Structure of Peridinin-Chlorophyll-Protein reconstituted with Chl-d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.580, 81.990, 75.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.110 - 1.800 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iis |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.544 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.110 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.150 | 0.570 |
| Number of reflections | 20022 | |
| <I/σ(I)> | 16.6 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1M CDCL2, 0,1M SODIUM ACETATE PH 4.6, 20-24% PEG 400, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 296K |
