2X1Q
Gelsolin Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.908, 63.425, 50.010 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.060 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.100 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 92321 | |
| <I/σ(I)> | 15.2 | 3.78 |
| Completeness [%] | 91.3 | 77.8 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 1000, 100 MM PHOSPHATE/ CITRATE PH 4.4, 100 MM LISO4. |
