2X1O
Gelsolin Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.393, 60.393, 78.242 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.340 |
| R-factor | 0.21277 |
| Rwork | 0.211 |
| R-free | 0.24007 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.340 |
| Rmerge | 0.070 |
| Number of reflections | 59490 |
| Completeness [%] | 87.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 1000, 100 MM PHOSPHATE/CITRATE PH 4.4, 100 MM LISO4 |
