2X1K
H71S mutant of the antibiotic resistance protein NimA from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.899, 38.969, 59.792 |
| Unit cell angles | 90.00, 113.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.23427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3o |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.733 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 29814 | |
| <I/σ(I)> | 0 | 0 |
| Completeness [%] | 96.7 | 79.8 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 13MG/ML PROTEIN, 0.6M SODIUM ACETATE, 0.1M BUFFER PH 6 (CITRATE/MES). |
