2X0X
Ribonucleotide reductase R1 subunit of E. coli to 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 223.877, 223.877, 336.467 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 169.031 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.311 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.080 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 142954 | |
| <I/σ(I)> | 15.79 | 3.19 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.48 | 7.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | LITHIUM SULPHATE APP 1.5M, SODIUM CITRATE BUFFER PH APP 6. |
