2X0R
R207S, R292S Mutant of Malate Dehydrogenase from the Halophilic Archeon Haloarcula marismortui (HoloForm)
Replaces: 1GT2Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | NONIUS |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-21 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.360, 125.910, 125.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.504 - 2.915 |
| R-factor | 0.1888 |
| Rwork | 0.184 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hlp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.401 |
| Data reduction software | XDS |
| Data scaling software | BIOMOL |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 3.010 |
| High resolution limit [Å] | 2.910 | 2.900 |
| Rmerge | 0.100 | 0.270 |
| Number of reflections | 20001 | |
| <I/σ(I)> | 15.5 | 4.5 |
| Completeness [%] | 97.8 | 93.1 |
| Redundancy | 5.03 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 2 M NACL, 25 MM TRIS PH 7.6, 2.5 MM NADH, 50% MPD |
