2X0O
Apo structure of the Alcaligin biosynthesis protein C (AlcC) from Bordetella bronchiseptica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.667, 128.910, 48.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.901 - 2.400 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ffe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.725 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 23068 | |
| <I/σ(I)> | 17.1 | 3.3 |
| Completeness [%] | 97.9 | 99.1 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.4M MG-SULFATE, 0.1M NA-CACODYLATE, PH6.0, 0.1M LI-SULFATE |
