2X08
cytochrome c peroxidase: ascorbate bound to the engineered ascorbate binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.000, 74.490, 106.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.010 |
| R-factor | 0.20148 |
| Rwork | 0.201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v23 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.637 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.350 |
| Number of reflections | 25406 | |
| <I/σ(I)> | 14.1 | 1.7 |
| Completeness [%] | 97.3 | 98.5 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | DIALYSIS AGAINST PHOSPHATE BUFFER 50 MM PH 6, MPD 30% |
