2X07
cytochrome c peroxidase: engineered ascorbate binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.000, 74.490, 106.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.840 - 1.860 |
| R-factor | 0.21357 |
| Rwork | 0.212 |
| R-free | 0.24693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v23 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.865 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.130 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.270 |
| Number of reflections | 31259 | |
| <I/σ(I)> | 18 | 3.4 |
| Completeness [%] | 90.4 | 92.3 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | DIALYSIS AGAINST PHOSPHATE BUFFER 50 MM PH 6, MPD 30% |
