2WZL

The Structure of the N-RNA Binding Domain of the Mokola virus Phosphoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	25.840, 48.870, 85.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.750 - 2.100
R-factor	0.2226
Rwork	0.220
R-free	0.26597
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyi
RMSD bond length	0.011
RMSD bond angle	1.178
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.170	0.580
Number of reflections	6812
<I/σ(I)>	9.9	1.7
Completeness [%]	99.6	97.9
Redundancy	5.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		30% (VOL/VOL) PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH), 50 MM AMMONIUM SULFATE, AND 100 MM BIS-TRIS, PH 6.5