2WZG
Legionella glucosyltransferase (Lgt1) crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 73 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.750, 122.750, 103.416 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.20081 |
| Rwork | 0.200 |
| R-free | 0.26175 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.381 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 45918 | |
| <I/σ(I)> | 29 | 5.8 |
| Completeness [%] | 99.2 | 1 |
| Redundancy | 10.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CRYSTALS WERE GROWN IN 0.1 M MGCL2, 12% PEG 6000, 0.1 M ACETAMIDO IMINODIACETIC ACID, PH 6.5. 2-METHYL-2,4-PENTANEDIOL (MPD) AT 20% IN MOTHER LIQUOR WAS USED AS A CRYOPROTECTANT. |
