2WZE
High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 173.037, 173.037, 136.159 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.020 - 2.500 |
| R-factor | 0.1489 |
| Rwork | 0.147 |
| R-free | 0.18920 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.019 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (SOLVE RESOLVE) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.100 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 81445 | |
| <I/σ(I)> | 18.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 292 | 0.1 M NA CITRATE, PH 5.6, 1.0M (NH4)H2PO4 AT 292 K. |
