2WZC
The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with ADP, 3PG and aluminium tetrafluoride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-22 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.220, 91.920, 108.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.16584 |
Rwork | 0.164 |
R-free | 0.19763 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c39 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.566 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.300 |
Number of reflections | 61523 | |
<I/σ(I)> | 11.4 | 3 |
Completeness [%] | 97.4 | 96.2 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 22% PEG2000MME, 0.1M BIS/TRIS PH 6.5 |