2WZA
Two intramolecular isopeptide bonds are identified in the crystal structure of the Streptococcus gordonii SspB C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.750, 80.150, 87.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.578 - 2.082 |
| R-factor | 0.1956 |
| Rwork | 0.192 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.408 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 21137 | |
| <I/σ(I)> | 17.77 | 6.3 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.09 | 6.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M CACL2, 0.1 M HEPES, PH 7.5, 20% (W/V) PEG4000. |
