2WZ1
STRUCTURE OF THE CATALYTIC DOMAIN OF HUMAN SOLUBLE GUANYLATE CYCLASE 1 BETA 3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.590, 90.380, 140.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 1.630 |
| R-factor | 0.18536 |
| Rwork | 0.184 |
| R-free | 0.21922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3et6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.585 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 49220 | |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PROTEIN WAS CONCENTRATED TO 12 MG/ML IN PURIFICATION BUFFER (10MM HEPES, 500MM NACL, 5% GLYCEROL, 10% GALACTOSE, 0.5MM TCEP)AND SET UP IN SITTING DROP IN A 1:2 RATIO WITH HAMPTON INDEX SCREEN - B8 (1.4M TRI-SODIUM CITRATE DIHYDRATE, 0.1M HEPES, PH 7.5). |
