2WZ0
L38V SOD1 mutant complexed with aniline.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.006, 68.261, 50.988 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.720 |
| R-factor | 0.19767 |
| Rwork | 0.194 |
| R-free | 0.25973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9v |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.746 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 25520 | |
| <I/σ(I)> | 15 | 2.1 |
| Completeness [%] | 98.3 | 84.7 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 150 MM NACL,3.0 M AMMONIUM SULPHATE, 100 MM TRIS-HCL PH 7.5 |
