2WYU
High resolution structure of Thermus thermophilus enoyl-acyl carrier protein reductase apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.270, 107.800, 85.980 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 14.960 - 1.500 |
| R-factor | 0.17037 |
| Rwork | 0.170 |
| R-free | 0.19972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ulu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.467 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 151147 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 96.4 | 83.8 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 20% (W/V) POLYETHYLENE GLYCOL 4000, 100 MM SODIUM CITRATE AT PH 5.4 |
