2WYI
Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase complexed with swainsonine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 178.680, 178.680, 198.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 132.450 - 2.600 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| Starting model (for MR) | 2wyh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.111 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 93181 | |
| <I/σ(I)> | 27.7 | 4.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15 MG/ML 3C CLEAVED SPGH38 MIXED WITH 3% V/V GLYCEROL, 54% V/V TACSIMATE (PH 7.0) AND 2% V/V POLYETHYLENE GLYCOL 6000 |
