2WY7
Staphylococcus aureus complement subversion protein Sbi-IV in complex with complement fragment C3d revealing an alternative binding mode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.430, 81.010, 87.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.780 - 1.700 |
| R-factor | 0.17377 |
| Rwork | 0.172 |
| R-free | 0.20488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.231 |
| Data reduction software | HKL-2000 |
| Data scaling software | DENZO |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 41186 | |
| <I/σ(I)> | 96 | 3710 |
| Completeness [%] | 100.0 | 87.6 |
| Redundancy | 9.5 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS PH8.0, 200MM NACL, 20%(W/V) PEG 4000 |
