2WY6
Clostridium perfringens alpha-toxin strain NCTC8237 mutant T74I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 107.238, 107.238, 225.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.914 - 3.200 |
| R-factor | 0.2221 |
| Rwork | 0.219 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CA1 WITHOUT RESIDUES 75-84 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.138 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.740 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.540 |
| Number of reflections | 22436 | |
| <I/σ(I)> | 22.4 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 1 M NA ACETATE, 0,1 M NA-HEPES, PH 7.6, 0.05 M CDSO4 |
