2WY4
Structure of bacterial globin from Campylobacter jejuni at 1.35 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.550, 37.980, 43.760 |
| Unit cell angles | 90.00, 102.68, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.350 |
| R-factor | 0.17997 |
| Rwork | 0.178 |
| R-free | 0.20887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.300 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 24978 | |
| <I/σ(I)> | 11.7 | 2.1 |
| Completeness [%] | 95.0 | 89 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.8M A.S.,0.1M TRIS/HCL, PH8.0 |
