2WXX
Crystal structure of mouse angiotensinogen in the oxidised form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.367, 99.416, 425.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 213.200 - 2.950 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 213.200 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.010 | 0.520 |
| Number of reflections | 36592 | |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 85.0 | 66 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 18-25% PEG4000, 0.2NH4F, 0.1M TRIS-HCL, PH8 |
