2WXX
Crystal structure of mouse angiotensinogen in the oxidised form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-01 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.367, 99.416, 425.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 213.200 - 2.950 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.27100 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 0.977 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 213.200 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.010 | 0.520 |
Number of reflections | 36592 | |
<I/σ(I)> | 9.2 | 1.5 |
Completeness [%] | 85.0 | 66 |
Redundancy | 2.6 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 18-25% PEG4000, 0.2NH4F, 0.1M TRIS-HCL, PH8 |