2WX3
Asymmetric trimer of the human DCP1a C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0688, 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.750, 64.750, 103.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.080 - 2.310 |
| R-factor | 0.21007 |
| Rwork | 0.208 |
| R-free | 0.25232 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.310 |
| Number of reflections | 11317 | |
| <I/σ(I)> | 11.9 | 2.5 |
| Completeness [%] | 98.9 | 95.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 50MM MES (PH 6.0), 1.2M NA-MALONATE |
