2WWY
Structure of human RECQ-like helicase in complex with a DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.333, 174.218, 100.229 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | 43.380 - 2.900 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.990 |
| Number of reflections | 44075 | |
| <I/σ(I)> | 14.8 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M SOUDIUM SULPHATE, 20% PEG3350, 10% ETHYLENE GLYCOL, PH 7.5 |
