2WWU
Crystal structure of the catalytic domain of PHD finger protein 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 150.980, 150.980, 150.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.760 - 2.150 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yu1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.750 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.810 |
| Number of reflections | 31194 | |
| <I/σ(I)> | 16.6 | 2 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 9.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.5 M (NH4)2SO4, 0.1 M SODIUM ACETATE PH 4.25 |
