2WWT
Intracellular subtilisin precursor from B. clausii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 121.640, 121.640, 106.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 105.570 - 2.680 |
| R-factor | 0.17 |
| Rwork | 0.166 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yjc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.905 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 2.830 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.140 | 0.700 |
| Number of reflections | 49303 | |
| <I/σ(I)> | 11.6 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BISTRIS, PH6.5, 0.1 M NACL, 1.5M AMMONIUM SULFATE |
