2WWC
3D-structure of the modular autolysin LytC from Streptococcus pneumoniae in complex with synthetic peptidoglycan ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.540, 68.800, 75.640 |
| Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
| Resolution | 41.670 - 1.750 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ww5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 57956 | |
| <I/σ(I)> | 14 | 2.1 |
| Completeness [%] | 98.0 | 84 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
