2WW5
3D-structure of the modular autolysin LytC from Streptococcus pneumoniae at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.800, 69.380, 75.870 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 72.930 - 1.610 |
| R-factor | 0.18477 |
| Rwork | 0.183 |
| R-free | 0.20417 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.077 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.930 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 76161 | |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 98.7 | 93.6 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
